Novel Coronavirus (SARS-CoV-2) IgG / IgM Antibody Test Kit (Immunochromatography)
This product is used for qualitative detection of IgG and IgM antibodies of SARS-COV-2 in human serum, plasma, whole blood and peripheral blood in vitro.
SARS-COV-2 belongs to the genus Coronavirus and the family Coronaviridae, which is divided into three genera: α, β and γ. Alpha and beta gene are only pathogenic to mammals, and gamma gene mainly causes bird infections. CoV is mainly transmitted through direct contact with secretions or through aerosols and droplets. There is also evidence that it can be transmitted through the fecal-oral route.
So far, there are 7 types of human coronaviruses (HCoV) that cause human respiratory diseases: HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, SARS-CoV, MERS-CoV and new coronaviruses (2019), which are important pathogens of human respiratory infections. Among them, SARS-COV-2 was found in the Wuhan Viral Pneumonia cases in 2019, with clinical symptoms of fever, fatigue, cough and others, accompanied by dyspnea. This infection can rapidly develop into severe pneumonia, respiratory failure, and acute respiratory vesicles syndrome, septic shock, multiple organ failure, severe acid-base metabolism disorders, etc., and may cause death.
This kit uses immunochromatography. The test card contains: 1) recombinant SARS-COV-2 antigens and quality control antibodies, and both are labeled with colored microspheres; 2) two detection lines (G and M lines) and a quality control line (C line) are fixed on nitrocellulose membrane, M line is fixed with monoclonal anti-human IgM antibody for detecting SARS-COV-2 IgM antibody, G line is fixed with monoclonal anti-human IgG antibody for detecting SARS-COV-2 IgG antibody, and C-line is fixed with quality control antibody.
When an appropriate amount of the test sample is removed to the sample hole of the test card, the sample will move forward along the test card with the reaction of capillary. If the sample contains IgM antibody, the antibody will bind to the colored microsphere-labeled SARS-COV-2 antigen. The immune complex will be captured by the anti-human IgM antibody immobilized on the membrane and form a red M line, which represents a positive test result of SARS-COV-2 IgM antibody test.
If the sample contains IgG antibody, the antibody will bind to the colored microsphere-labeled SARS-COV-2 antigen, and the immune complex will be captured by the antibody immobilized on the membrane and form a red G line, which indicates a positive test result of SARS-COV-2 IgG antibody test.
If neither the G line nor the M line shows color, the test result is negative. The card also contains a quality control line C line. No matter G or M detection line shows color or not, the quality control C line in red should always be visible. The quality control line is the color band of the quality control antibody immune complex. If the C line does not appear, it indicates that the test result is invalid, and thus a re-test is required with an unused test card.
1. Test card: binding pad (SARS-COV-2 antigen labeled with red latex microspheres, rabbit IgG labeled with red latex microspheres), nitrocellulose membrane (C line coated with goat anti-rabbit IgG polyclonal antibody, G line coated with anti-human IgG Monoclonal antibody, M line coated with mouse anti-human IgM monoclonal antibody), hemofiltration pad, sample pad, suction pad and PVC plate.
2. Disposable plastic pipette (sample dropper)
4. Sample diluent: 20Mm PBS Ph7.4 ± 0.5
5. Packaging box and instruction manual.